ABSTRACT
Objective To study the chemical constituents from the stems of Uncaria lancifolia. Methods The isolation and purification were carried out by silica gel column chromatography, RP18, Sephadex LH-20, and preparative HPLC. The structures of the isolated compounds were elucidated by physical and chemical properties, and spectroscopic methods. Results Eighteen compounds were isolated and identified from 95% ethanol extract from the stems of U. lancifolia and characterized as uncarine A (1), uncarine E (2), isomitraphylline (3), tetrahydroalstonine (4), strictosidine (5), cadambine (6), glabratine (7), strictosamide (8), (13R)-hydroxy- octodeca-(9Z,11E,15Z)-trien-oic acid (9), (6S,9R)-roseoside (10), periplanetin (11), integracin A (12), integracin B (13), 6β,19α- dihydroxyurs-3-oxours-12-en-28-oic aicd (14), ursolic acid (15), oleanic acid (16), β-sitosterol (17), and β-daucosterol (18). Conclusion This is the first report for the chemical constituents from U. lancifolia. All compounds are obtained from this plant for the first time, and compounds 9-13 are isolated from Uncaria genus for the first time. Compounds 1-8 are monoterpene indole alkaloids, which are characteristic constituents in Uncaria genus.
ABSTRACT
Objective: To study the chemical constituents in the aerial parts of Euphorbia royleana. Methods: The constituents were isolated and purified by chromatographic of silica gel, Sephadex LH-20, RP18, and MCI columns, and their structures were elucidated by spectroscopic analyses. Results: Twelve compounds were isolated from the 70% acetone extracts in the aerial parts of E. royleana and their structures were identified as (6S, 9R)-roseoside (1), 13-carboxyblumenol C 9-O-β-glucoside (2), 3, 3'-dimethylellagic acid-4-O-β-D-glucopyranoside (3), cycloart-23-ene-3β, 25-diol (4), 23(E)-25-methoxycycloart-23-en-3β-ol (5), α-amyrin (6), triptohypol F (7), 9(11), 12-dieneoleana-3β-ol (8), friedelane-3β, 29-diol (9), D:A-friedoolean-29-ol-3-one (10), dischidiol (11), and lupeol (12). Conclusion: Compounds 1, 2, 7-11 are obtained from the plants in Euphorbia L. for the first time and compounds 3-5 and 12 are isolated from this plant for the first time.